Show simple item record Spies, Fernanda Sá Ribeiro, Andrezza Wolowski Soares, Daniela Fernandes Ramos Ribeiro, Marta Osório Martin, Anandi Palomino, Juan Carlos Rossetti, Maria Lucia Rosa Silva, Pedro Eduardo Almeida da Zaha, Arnaldo 2013-01-25T22:58:34Z 2013-01-25T22:58:34Z 2011
dc.identifier.citation SPIES, Fernanda Sá et al. Streptomycin resistance and lineage specific polymorphisms in Mycobacterium tuberculosis gidB gene. Journal of Clinical Microbiology, v. 49, n. 7, p. 2625-2630, 2011. Disponível em: <>. Acesso em: 16 set. 2012. pt_BR
dc.description.abstract Mutations related to streptomycin resistance in the rpsL and rrs genes are well known and can explain about 70% of this phenotypic resistance. Recently, the gidB gene was found to be associated with low-level streptomycin resistance in Mycobacterium tuberculosis. Mutations in gidB have been reported with high frequency, and this gene appears to be very polymorphic, with frameshift and point mutations occurring in streptomycinsusceptible and streptomycin-resistant strains. In this study, mutations in gidB appeared in 27% of streptomycin- resistant strains that contained no mutations in the rpsL or rrs genes, and they were associated with low-level streptomycin resistance. However, the association of certain mutations in gidB with streptomycin resistance needs to be further investigated, as we also found mutations in gidB in streptomycin-susceptible strains. This occurred only when the strain was resistant to rifampin and isoniazid. Two specific mutations appeared very frequently in this and other studies of streptomycin-susceptible and -resistant strains; these mutations were not considered related to streptomycin resistance, but as a polymorphism. We stratified the strains according to the different phylogenetic lineages and showed that the gidB16 polymorphism (16G allele) was exclusively present in the Latin American-Mediterranean (LAM) genotype, while the gidB92 polymorphism(92C allele) was associated with the Beijing lineage in another population. In the sample studied, the two characterized single-nucleotide polymorphisms could distinguish LAM and Beijing lineages from the other lineages. pt_BR
dc.language.iso eng pt_BR
dc.rights open access pt_BR
dc.title Streptomycin resistance and lineage specific polymorphisms in Mycobacterium tuberculosis gidB gene pt_BR
dc.type article pt_BR
dc.identifier.doi 10.1128/JCM.00168-11 pt_BR

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