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dc.contributor.author Martin, Anandi
dc.contributor.author Groll, Andrea Von
dc.contributor.author Fissette, Krista
dc.contributor.author Palomino, Juan Carlos
dc.contributor.author Varaine, Francis
dc.contributor.author Portaels, Françoise
dc.date.accessioned 2013-01-28T14:14:52Z
dc.date.available 2013-01-28T14:14:52Z
dc.date.issued 2008
dc.identifier.citation MARTIN, Anandi et al. Rapid detection of mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system. Journal of Clinical Microbiology, v. 46, n. 12, p. 3952-3956, 2008. Disponível em:<http://jcm.asm.org/content/46/12/3952.full.pdf+html>. Acesso em: 16 set. 2012. pt_BR
dc.identifier.uri http://repositorio.furg.br/handle/1/3136
dc.description.abstract The objective of this study was to evaluate the manual mycobacterium growth indicator tube (MGIT) system for the testing of Mycobacterium tuberculosis susceptibility to second-line drugs compared to the proportion method. One hundred eighty-eight M. tuberculosis isolates were tested for susceptibility to ofloxacin, kanamycin, ethionamide, and capreomycin by the manual MGIT, and results were compared to those obtained with the proportion method on 7H11 agar, considered a reference method. Results for ofloxacin and capreomycin were excellent, with 100% accuracy, and a result of 99.4% accuracy was achieved for kanamycin. For ethionamide,accuracy was lower, with a result of 86.7% compared to that of the proportion method. We proposed the following critical concentrations for the drugs: for ofloxacin, 2.0 g/ml; for kanamycin, 2.5 g/ml; for ethionamide, 5 g/ml; and for capreomycin, 2.5 g/ml. The time required to obtain results was an average of 8 days by the manual MGIT and 3 weeks by the reference method. Our results show that the manual MGIT is an accurate method for the rapid susceptibility testing of M. tuberculosis to second-line drugs. There is no need for a machine when using the manual MGIT, and results can be read with a simple UV lamp or with a semiquantitative reader, which considerably reduces the cost of the method. pt_BR
dc.language.iso eng pt_BR
dc.rights open access pt_BR
dc.title Rapid detection of mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system pt_BR
dc.type article pt_BR
dc.identifier.doi 10.1128 pt_BR


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