Microcystin-induced oxidative stress in Laeonereis acuta (Polychaeta, Nereididae)

Abstract

Oxidative stress induced by microcystins was evaluated in an estuarine worm, Laeonereis acuta (Nereididae).Ten organisms were exposed to lyophilized cells of a toxic Microcystis aeruginosa strain RST9501 ( 2 lg/mL microcystins, MC); 10 were exposed to lyophilized cells of a nontoxic Aphanotece sp. strain RSMan92 and 10 were maintained without cyanobacterial cells. Exposure time was 48 h. The enzymatic antioxidant defenses, as well as the oxidative damage, were analyzed. Toxic and nontoxic cyanobacteria lowered catalase activity with no changes in glutathione reductase and glutathione-S-transferase activities. This may have led to toxin intracellular accumulation, which should favor oxidative stress generation,observed by the high lipid peroxide and DNA–protein crosslink levels in the group exposed to MC.