Growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

Groll, Andrea Von; Martin, Anandi; Portaels, Françoise; Silva, Pedro Eduardo Almeida da; Palomino, Juan Carlos


We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.

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  • FAMED – Artigos publicados em periódicos