Show simple item record Borsuk, Sibele Dellagostin, Marina Mírian Madeira, Suselaine de Góes Lima, Crislaine Boffo, Maria Marta Santos Mattos, Ivo Gomes de Silva, Pedro Eduardo Almeida da Dellagostin, Odir Antonio 2012-10-15T12:14:32Z 2012-10-15T12:14:32Z 2005
dc.identifier.citation BORSUK, Sibele et al. Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with high incidence of tuberculosis. Microbes and Infection, v. 7, n. 13, p. 1338-1344, 2005. Disponível em: <>. Acesso em: 18 set. 2012. pt_BR
dc.identifier.issn 1286-4579
dc.description.abstract One hundred and seventy Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results were compared to the World Spoligotyping Database (Institut Pasteur de Guadeloupe), which contains data from> 14,000 worldwide isolates of M. tuberculosis. The isolates clustered by spoligotyping were further characterized by IS6110-RFLP to confirm the clonal relationship. Sixty-six different spoligotypes were identified, grouping 125 of the isolates (74%). Approximately half of the isolates belonged to seven of the most frequently occurring spoligotypes in the database. Three shared types (with two or more isolates) not previously identified were given the type numbers 826, 827 and 863. An additional 45 spoligotypes were identified that did not match any existing database pattern. RFLP characterization reduced the number of isolates in most of the clusters, thereby showing a higher differentiation capacity than spoligotyping. These results highlight the importance of molecular epidemiology studies of tuberculosis in insufficiently studied regions with a high TB burden, in order to uncover the true extent of genetic diversity of the pathogen. pt_BR
dc.language.iso eng pt_BR
dc.rights restrict access pt_BR
dc.subject IS6110-RFLP pt_BR
dc.subject Spoligotyping pt_BR
dc.subject Molecular epidemiology pt_BR
dc.title Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with high incidence of tuberculosis pt_BR
dc.type article pt_BR
dc.identifier.doi 10.1016/j.micinf.2005.05.009 pt_BR

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