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dc.contributor.author Barros, Lélia Pacheco Corrêia
dc.contributor.author Monserrat, José María
dc.contributor.author Yunes, João Sarkis
dc.date.accessioned 2013-09-16T17:29:45Z
dc.date.available 2013-09-16T17:29:45Z
dc.date.issued 2004
dc.identifier.citation BARROS, Lélia Pacheco Corrêia; MONTSERRAT, José Maria; YUNES, João Sarkis. Determination of optimized protocols for the extraction of anticholinesterasic compounds in environmental samples containing cyanobacteria species. Environmental Toxicology and Chemistry, v. 23, n. 4, p. 883-889, 2004. Disponível em: <http://www.ncbi.nlm.nih.gov/pubmed/15095883>. Acesso em: 08 jul. 2012. pt_BR
dc.identifier.issn 1552-8618
dc.identifier.uri http://repositorio.furg.br/handle/1/3789
dc.description.abstract The effects of temperature and pH during extraction of toxin(s) from the cyanobacterial genus Anabaena were studied. Different extracts were compared for their anticholinesterase potency against purified eel acetylcholinesterase. A lyophilized powder from Anabaeba spiroides cells was dissolved in ethanol, sonicated, and filtered. This solution was then evaporated at three different temperatures (40, 60, or 808C), resuspended in chloroform, and washed in deionized water at three different pH values (3.3, 5.0, or 8.5). At 408C, the estimated 50% inhibition concentration (IC50) was 2.06, 2.19, and 8.64 mg lyophilized A. spiroides cells ml21 for the extraction pH of 3.3, 5.0, and 8.5, respectively. At 608C, the values were 4.82, 3.06, and 12.0 mg/ml at increasing pH values. Finally, at 808C, the IC50 values were 3.52, 4.35, and 30.1 mg/ml at the same pH values. These results indicate that pH 3.3 and a temperature of 408C should be employed to preserve toxin content. The optimized protocol was employed to evaluate the anticholinesterase potency of environmental samples of water containing different numbers of filaments of cyanobacterial species. Registered inhibition ranged from 5.11 to 11.1%, supporting the feasibility of the method. pt_BR
dc.language.iso eng pt_BR
dc.rights restrict access pt_BR
dc.subject Anabaena spiroides pt_BR
dc.subject Acetylcholinesterase pt_BR
dc.subject Neurotoxins pt_BR
dc.subject Inhibition constants pt_BR
dc.title Determination of optimized protocols for the extraction of anticholinesterasic compounds in environmental samples containing cyanobacteria species pt_BR
dc.type article pt_BR


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