Possible role of non-classical chromathophorotropins on the regulation of the crustacean erythrophore

Abstract

Two neuropeptides, the pigment dispersing hormone (PDH) and the pigment concentrating hormone (PCH), are well known to respectively promote centrifugal and centripetal granule translocation in the freshwater shrimp Macrobrachium potiuna erythrophores. Herein,we demonstrate for the first time the effects of crustacean non-classical chromatophorotropins on the pigment migration in M. potiuna erythrophores. Although proctolin, 20-hydroxyecdisone (20HE),and melatonin were ineffective, the crustacean cardioactive peptide (CCAP) was a full agonist,inducing pigment dispersion in a dose-dependent manner with EC50 of 9.5 · 10–7 M. In addition, concentrations of CCAP lower than the minimal effective dose (10–8 and 10–7 M) decreased the PCH-induced aggregation, shifting rightward the dose-response curve (DRC) to PCH 2.2- and 29-fold, respectively. Surprisingly, melatonin (10–7 and 10–6 M) also shifted to the right 8.7- and 46.5- fold, respectively, the DRC to PCH. In conclusion, our data demonstrate that besides PCH and PDH, CCAP and melatonin also regulate the pigment migration within the crustacean erythrophore.