Detection of Helicobacter pylori by phenotypic and genotypic methods

Fonseca, Tesiê Leopoldo; Moraes, Ernani Pinho de; Juliano, Carlos Renan Varela; Azambuja, Ana Maria Volkmer de; Scaini, Carlos James; Sassi, Raul Andrés Mendonza; Silva, Pedro Eduardo Almeida da


Aims This research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in solated strains that were positive for ureA by PCR positive. Results Sensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples. Conclusions In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.

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  • EE - Artigos Publicados em Periódicos