Isolation and characterization of 18 microsatellites for Lycengraulis grossidens (Pisces: Clupeiformes)
Mai, Ana Cecilia Giacometti; Vieira Sobrinho, João Paes; Plavan, Alicia Addis Acuña; Nobrega, Marcelo Francisco de; Moraes, Leonardo Evangelista; Rodrigues, Fábio Lameiro; Marins, Luis Fernando Fernandes
Abstract:
Twelve polymorphic and six non-polymorphic microsatellite loci were isolated and characterized from a CT–GT enriched genomic library of Lycengraulis grossidens. The number of alleles ranged from 1 to 29. The observed and expected heterozygosity ranged from 0.19 to 0.8 and from 0.18 to 0.95, respectively. Cross-species amplification of the markers developed for L. grossidens was successful in the wild Engraulididae species Anchoa spinifer, Anchoa marinii, Anchoviella lepidentostole, Cetengraulis edentulous and Engraulis anchoita. The developed microsatellite loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow for these important food sources.