dc.contributor.author |
Spies, Fernanda Sá |
|
dc.contributor.author |
Groll, Andrea Von |
|
dc.contributor.author |
Ribeiro, Andrezza Wolowski |
|
dc.contributor.author |
Soares, Daniela Fernandes Ramos |
|
dc.contributor.author |
Ribeiro, Marta Osório |
|
dc.contributor.author |
Costa, Elis Regina Dalla |
|
dc.contributor.author |
Martin, Anandi |
|
dc.contributor.author |
Palomino, Juan Carlos |
|
dc.contributor.author |
Rossetti, Maria Lucia |
|
dc.contributor.author |
Zaha, Arnaldo |
|
dc.contributor.author |
Silva, Pedro Eduardo Almeida da |
|
dc.date.accessioned |
2017-03-01T16:48:32Z |
|
dc.date.available |
2017-03-01T16:48:32Z |
|
dc.date.issued |
2012 |
|
dc.identifier.citation |
SPIES, Fernanda Sá et al. Biological cost in Mycobacterium tuberculosis with mutations in the rpsL, rrs, rpoB, and katG genes. Tuberculosis, v 93, n. 2, p. 150–154. Disponível em: <https://www.researchgate.net/publication/234019090_Biological_cost_in_Mycobacterium_tuberculosis_with_mutations_in_the_rpsL_rrs_rpoB_and_katG_genes>. Acesso em: 27. fev. 2017. |
pt_BR |
dc.identifier.issn |
1472-9792 |
|
dc.identifier.uri |
http://repositorio.furg.br/handle/1/7083 |
|
dc.description.abstract |
When bacteria develop drug-resistant mutations, there is often an associated biological cost; however,
some strains can exhibit low- or no-cost mutations. In the present study, a quantitative resazurin
reduction assay was used to measure the biological cost of Mycobacterium tuberculosis isolates that
contained different mutations in the rpsL, rrs, rpoB, and katG genes, and showed different resistance
profiles. Biological costs were determined by comparing the growth curves of drug-resistant isolates
with drug-susceptible strains. Some strains, such as those with rpoB mutations other than S531L and
strains with mutations in all of the studied genes, grew more slowly than did drug-susceptible strains.
However, some strains grew more quickly than drug-susceptible strains, such as those that had only the
rpsL K43R mutation. Strains with the mutation katG S315T presented heterogeneous biological costs.
When analyzed individually, strains with the mutations rpsL43/katG315, rpoB531, and rpoB531/katG315
grew faster than drug-susceptible strains. The results suggest that some strains with the most common
mutations correlated to a high resistance toward streptomycin, isoniazid and rifampicin can grow as well
as or better than susceptible strains. |
pt_BR |
dc.language.iso |
eng |
pt_BR |
dc.rights |
restrict access |
pt_BR |
dc.subject |
Biological cost |
pt_BR |
dc.subject |
M. tuberculosis |
pt_BR |
dc.subject |
MDR strains |
pt_BR |
dc.subject |
Resazurin reduction assay |
pt_BR |
dc.subject |
Clinical isolates |
pt_BR |
dc.title |
Biological cost in Mycobacterium tuberculosis with mutations in the rpsL, rrs, rpoB, and katG genes |
pt_BR |
dc.type |
article |
pt_BR |
dc.identifier.doi |
10.1016/j.tube.2012.11.004 |
pt_BR |