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dc.contributor.author Monserrat, José María
dc.contributor.author Bianchini, Adalto
dc.contributor.author Bainy, Afonso Celso Dias
dc.date.accessioned 2011-06-17T18:31:08Z
dc.date.available 2011-06-17T18:31:08Z
dc.date.issued 2002
dc.identifier.citation MONSERRAT, J. M.; BIANCHINI, A.; BAINY, A.C.D.. Kinetic and toxicological characteristics of acetylcholinesterase from the gills of oysters (Crassostrea rhizophorae) and other aquatic species. Marine Environmental Research, Inglaterra, v. 54, n. 3-5, p. 781-785, 2002. Disponível em: <http://www.sciencedirect.com.ez40.periodicos.capes.gov.br/science?_ob=MImg&_imagekey=B6V7H-45W35TF-C-4&_cdi=5843&_user=923856&_pii=S0141113602001368&_origin=browse&_zone=rslt_list_item&_coverDate=12%2F31%2F2002&_sk=999459996&wchp=dGLbVlz-zSkzS&md5=3ae1643501e9feba714647af4a8924df&ie=/sdarticle.pdf>. Acesso em: 15 jun. 2011. pt_BR
dc.identifier.issn 0141-1136
dc.identifier.uri http://repositorio.furg.br/handle/1/751
dc.description.abstract The aim of this work was to characterize the cholinesterases from gills of Crassostrea rhizophorae in order to use them as biomarkers. Gills were homogenized and then centrifuged (9000_g, 4 _C, 30 min). S9 and Triton X-100 S9 treated (TX S9) fractions were employed as enzyme source. Kmap and Vmax were estimated, using acetylthiocholine iodide as substrate. Inhibition assays were performed with iso-OMPA and eserine. The Kmap for S9 and TX S9 fractions were 0.05 and 0.06 mM, whereas the Vmax were 1.92 and 5.84 nmol/min/mg protein, respectively. No inhibition was detected when the samples were incubated with iso-OMPA, suggesting the presence of acetylcholinesterases (AChE) in oyster gill homogenates. Sensitivity to eserine inhibition of AChE in the gills of oysters is intermediate when compared with other aquatic species. pt_BR
dc.language.iso eng pt_BR
dc.rights restrict access
dc.subject Oyster pt_BR
dc.subject Crassostrea rhizophorae pt_BR
dc.subject Cholinesterase pt_BR
dc.subject Inhibition pt_BR
dc.subject Eserine pt_BR
dc.subject Kinetic parameters pt_BR
dc.title Kinetic and toxicological characteristics of acetylcholinesterase from the gills of oysters (Crassostrea rhizophorae) and other aquatic species pt_BR
dc.type article pt_BR


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