Buffon, Jaqueline GardaBaraj, EdliraFurlong, Eliana Badiale2012-03-062012-03-062010BUFFON, Jaqueline Garda; BARAJ, Edlira; FURLONG, Eliana Badiale. Effect of deoxynivalenol and T-2 toxin in malt amylase activity. Brazilian archives of biology and technology, Curitiba, v. 53, n. 3, p. 505 -511, mai. -jun. 2010. Disponível em: <http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1516-89132010000300002&lng=en&nrm=iso&tlng=en> Acesso em: 14 Jan. 2012.1516-8913http://repositorio.furg.br/handle/1/1852The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the a-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the b-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a significant model for enzymatic activity of b-amylase.engopen accessAminolitic enzymesFermentationTrichothecenesBarleyarticlehttp://dx.doi.org/10.1590/S1516-89132010000300002