Buffon, Jaqueline GardaBaraj, EdliraFurlong, Eliana Badiale2014-07-222014-07-222010BUFFON, Jaqueline Garda; BARAJ, Edlira; FURLONG, Eliana Badiale. Effect of Deoxynivalenol and T-2 Toxin in Malt Amylase Activity. Brazilian Archives of Biology and Technology, v. 53, n. 3, p. 505-511, 2010. Disponível em: <http://www.scielo.br/pdf/babt/v53n3/a02v53n3.pdf>. Acesso em: 10 maio 2014.1516-8913http://repositorio.furg.br/handle/1/4441The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the a-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the b-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a significant model for enzymatic activity of b-amylase.engopen accessAminolitic enzymesfermentationtrichothecenesbarleyarticle