Santos, Elisane Odriosolla dosMichelon, MarianoFurlong, Eliana BadialeBurkert, Janaina Fernandes de MedeirosKalil, Susana JulianoBurkert, Carlos André Veiga2014-08-222014-08-222012SANTOS, Elisane Odriosolla dos,et all. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. BRAZILIAN JOURNAL OF MICROBIOLOGY. São Paulo, v. 43, n. 2, p.432-440, São Paulo, Apr./Jun. 2012. Online. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200002#back>. Acesso em: 01 de jul. 2014.1517-8382http://repositorio.furg.br/handle/1/4506The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).engopen accessRaw GlycerolSingle Cell ProteinYeast BiomassYarrowia Lipolyticaarticledx.doi.org/10.1590/S1517-83822012000200002