Abstract:
OBJETIVO: Determinar a frequência do Papilomavírus Humano (HPV) na placenta, no colostro e no sangue do cordão
umbilical de parturientes e seus neonatos atendidos no Ambulatório de Ginecologia e Obstetrícia do Hospital Universitário
de Rio Grande (RS), Brasil. MÉTODOS: Foram coletadas biópsias de 150 placentas do lado materno, 150 do lado
fetal, 138 amostras do sangue do cordão umbilical e 118 amostras de colostro. As biópsias de placenta foram
coletadas da porção central e periférica. O DNA foi extraído segundo protocolo do fabricante e conforme referência
encontrada na literatura. O HPV foi detectado pela técnica da reação em cadeia da polimerase aninhada (PCR-Nested)
com os primers MY09/11 e GP5/GP6. A genotipagem foi por sequenciamento direto. As participantes responderam
a um questionário autoaplicado com dados demográficos e clínicos, a fim de caracterizar a amostra. RESULTADOS:
O HPV foi detectado em 4% (6/150) do lado materno das placentas, 3,3% (5/150) do lado fetal; 2,2% (3/138)
no sangue do cordão e 0,8% (1/118) no colostro. A taxa de transmissão vertical foi de 50%. O genótipo de baixo
risco oncogênico encontrado foi o HPV-6 (60%) e de alto risco, os HPV-16 e HPV-18 (20% cada). CONCLUSÕES:
Esses resultados sugerem que o HPV pode infectar a placenta, o colostro e o sangue do cordão umbilical.
PURPOSE: To determine the frequency of Human Papillomavirus (HPV) in the placenta, in the colostrum and in the
umbilical cord blood of parturient women and their newborns assisted at the Clinic of Gynecology and Obstetrics of the
University Hospital of Rio Grande (RS), Brazil. METHODS: Biopsies were collected from 150 placentas on the maternal
side, 150 on the fetal side, 138 samples of umbilical cord blood and 118 of the colostrum. The placenta biopsies were
collected from the central and peripheral portions. DNA was extracted according to the manufacturer’s protocol and
to a reference found in the literature. HPV was detected by the nested polymerase chain reaction (PCR-Nested) using
primers MY09/11 and GP5/GP6. Genotyping was performed by direct sequencing. The participants responded to
a self-applied questionnaire with demographic and clinical data, in order to characterize the sample. RESULTS: HPV
was detected in 4% (6/150) of cases on the mother’s side of the placentas, in 3.3% (5/150) on the fetal side, in
2.2% (3/138) in umbilical cord blood and in 0.84% (1/118) in colostrum samples. The vertical transmission rate was
50%. HPV-6 was the low-risk genotype found (60%) and the high-risk genotypes were HPV-16 and HPV-18 (20% each).
CONCLUSIONS: These results suggest that HPV can infect the placenta, the colostrum and the umbilical cord blood.
