Navegando por Autor "Cipolatti, Eliane Pereira"

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Application of protein-phenolic based coating on tomatoes (Lycopersicum esculentum)
(2012) Cipolatti, Eliane Pereira; Kupski, Larine; Rocha, Meritaine da; Oliveira, Melissa dos Santos; Buffon, Jaqueline Garda; Furlong, Eliana Badiale
The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum). Tests were performed with glycerol 3% (v/v), glycerol with protein-phenolic rice bran extract (5%), glycerol with protein-phenolic extract after 96 hours of fermentation (5%), and a control (without coating). The coated cherry tomatoes were kept at room temperature for 28 days. Mass loss, pH and acidity, total soluble solids, and carotenoids were determined every 96 hours. The coating made from the biomass extract reduced the carotenoid and acidity levels in the fruits studied by 17 and 21.1%, respectively, compared to the control. The coating proved an efficient barrier to water vapor with mass loss of 57% less than the control suggesting that it can be used as an alternative for vegetable tissue conservation.
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Changes in lipid, fatty acids and phospholipids composition of whole rice bran after solid-state fungal fermentation
(2011) Oliveira, Melissa dos Santos; Feddern, Vivian; Kupski, Larine; Cipolatti, Eliane Pereira; Furlong, Eliana Badiale; Soares, Leonor Almeida de Souza
The aim of this study was to evaluate fermented rice bran phospholipids, lipids and fatty acid content in a fermentation solid system with Rhizopus oryzae fungus. For this, aliquots were withdrawn every 24 h over 120 h. The content of phospholipids was determined by colorimetric method.Esterified fatty acids were separated by gas chromatography, then identified and quantified. The total lipids from fermented rice bran (FB) decreased from 20.4% to 11.2% in the range between 0 h and 120 h of fermentation while phospholipid contents were increased up to 2:4 mg P g 1 lipid . In fermented bran, oleic, palmitic and linoleic acids prevailed, with a decrease in saturated fatty acids (20%) and increase in the unsaturated ones (5%). This study showed that rice bran fermentation with R. oryzae can be applied to the production of phospholipids altering the saturated to unsaturated fatty acid ratio.
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Estudo das condições de extração de compostos fenólicos de cebola (Allium cepa L.)
(2009) Souza, Michele Moraes de; Recart, Vânia Machado; Rocha, Meritaine da; Cipolatti, Eliane Pereira; Furlong, Eliana Badiale
O objetivo deste trabalho foi estabelecer as melhores condições para efetuar extração de compostos fenólicos totais de diferentes classes comerciais de cebola (Allium cepa L.). Por meio de Planejamento Experimental Fatorial foram determinadas as variáveis que infl uenciam signifi cativamente nas etapas de extração. As variáveis estudadas foram: natureza do solvente, procedimento de agitação, tempo de extração e tempo de agitação com e sem interrupções. A melhor combinação resultou em um modelo preditivo, empregandose metanol como solvente, agitação de 120 minutos a 200 rpm. O maior conteúdo fenólico em diferentes classes de cebola foi 2275 μg/g, 88% de recuperação e o limite de quantifi cação foi de 31 μg fenóis/g.
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Solid-State Fermentation for the Enrichment and Extraction of Proteins and Antioxidant Compounds in Rice Bran by Rhizopus oryzae
(2012) Kupski, Larine; Cipolatti, Eliane Pereira; Rocha, Meritaine da; Oliveira, Melissa dos Santos; Soares, Leonor Almeida de Souza; Furlong, Eliana Badiale
The objective of this work was to evaluate the solid-state fermentation with Rhizopus oryzae CCT 7560 of rice bran for the enrichment of proteins and the antioxidant compounds in the fermented biomass. Fermentation was performed in tray bioreactors at 30ºC for 120 h. Protein extraction was done at alkaline pH, followed by precipitation with acetone. Phenolic compounds were extracted with cold methanol. The maximum protein was recovered from after 120 h (26.6%). The content of total phenolic compounds increased during the fermentation and was maximum after 96 h, which inhibited the DPPH radical by 87%. The promising characteristics of the protein and phenolic extracts of the biomass suggested the application in the coating composition for vegetal tissues preservation.