Navegando por Autor "Durante, Andrei José"

Agora exibindo 1 - 2 de 2

Item
Isolation and Characterization of a New Arthrospira Strain
(2008) Morais, Michele Greque de; Reichert, Carolina da Cruz; Dalcanton, Francieli; Durante, Andrei José; Marins, Luis Fernando Fernandes; Costa, Jorge Alberto Vieira
A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol mÐ2 sÐ1 according to a complete 32 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μmax = 0.22 dÐ1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol mÐ2 sÐ1 and the highest protein content (86.0% w/w).
Item
Simultaneous Cultivation of Spirulina platensis and the Toxigenic Cyanobacteria Microcystis aeruginosa
(2006) Costa, Jorge Alberto Vieira; Morais, Michele Greque de; Dalcanton, Francieli; Reichert, Carolina da Cruz; Durante, Andrei José
Mangueira Lagoon, located in the extreme south of Brazil, has water with physicochemical characteristics such as alkaline pH and carbonate levels propitious for the growth of the cyanobacterium Spirulina platensis. Previously published studies have shown that Mangueira Lagoon water supplemented with small quantities of carbon and nitrogen is suitable for S. platensis cultivation and can significantly reduce production costs. We studied mixed cultures of Spirulina platensis and the toxic cyanobacterium Microcystis aeruginosa using a 23 factorial design in which the three factors were the initial biomass concentration of S. platensis and M. aeruginosa and the type of culture medium (100% Zarrouk’s medium or 80% Mangueira Lagoon water plus 20% Zarrouk’s medium). The highest S. platensis maximum specific growth rate (μmax) occurred in the culture with the highest M. aeruginosa biomass concentration and when undiluted culture medium was used (μmax = 0.283 dÐ1). The highest M. aeruginosa specific death rate (k) was obtained in the presence of S. platensis (k = 0.555 dÐ1) and was independent of the initial M. aeruginosa biomass concentration and culture medium, demonstrating that S. platensis cultures are not susceptible to contamination by M. aeruginosa. The culture medium had no significant influence (p > 0.05) on S. platensis μmax values, indicating that production costs could be reduced by using a medium consisting of 80% Mangueira Lagoon water plus 20% Zarrouk’s medium.