Navegando por Autor "Vallochi, Adriana Lima"

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Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells
(Biochemical Society, 2011) Garcia, Michele Carrett Dias; Votto, Ana Paula de Souza; Filgueira, Daza de Moraes Vaz Batista; Almeida, Daniela Volcan; Vallochi, Adriana Lima; D'Oca, Marcelo Gonçalves Montes; Marins, Luis Fernando Fernandes; Trindade, Gilma Santos
ASA (acetylsalicylic acid) is an NSAID (non-steroidal anti-inflammatory drug). ASA has gained attention as a potential chemopreventive and chemotherapeutic agent for several neoplasms. The aim of this study was to analyse the possible antitumoural effects of ASA in two erythroleukaemic cell lines, with or without the MDR (multidrug resistance) phenotype. The mechanism of action of different concentrations of ASA were compared in K562 (non-MDR) and Lucena (MDR) cells by analysing cell viability, apoptosis and necrosis, intracellular ROS (reactive oxygen species) formation and bcl-2, p53 and cox-2 gene expression. ASA inhibited the cellular proliferation or induced toxicity in K562 and Lucena cell lines, irrespective of the MDR phenotype. The ASA treatment provoked death by apoptosis and necrosis in K562 cells and only by necrosis in Lucena cells. ASA also showed antioxidant activity in both cell lines. The bcl-2, p53 and cox-2 genes in both cell lines treated with ASA seem to exhibit different patterns of expression. However, normal lymphocytes treated with the same ASA concentrations were more resistant than tumoral cells. The results of this work show that both cell lines responded to treatment with ASA, demonstrating a possible antitumoral and anti-MDR role for this drug.
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Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells
(2011) Garcia, Michele Carrett Dias; Votto, Ana Paula de Souza; Filgueira, Daza de Moraes Vaz Batista; Almeida, Daniela Volcan; Vallochi, Adriana Lima; D'Oca, Marcelo Gonçalves Montes; Marins, Luis Fernando Fernandes; Trindade, Gilma Santos
ASA (acetylsalicylic acid) is an NSAID (non-steroidal anti-inﬂammatory drug). ASA has gained attention as a potential chemopreventive and chemotherapeutic agent for several neoplasms. The aim of this study was to analyse the possible antitumoural effects of ASA in two erythroleukaemic cell lines, with or without the MDR (multidrug resistance) phenotype. The mechanism of action of different concentrations of ASA were compared in K562 (non-MDR) and Lucena (MDR) cells by analysing cell viability, apoptosis and necrosis, intracellular ROS (reactive oxygen species) formation and bcl-2, p53 and cox-2 gene expression. ASA inhibited the cellular proliferation or induced toxicity in K562 and Lucena cell lines, irrespective of the MDR phenotype. The ASA treatment provoked death by apoptosis and necrosis in K562 cells and only by necrosis in Lucena cells. ASA also showed antioxidant activity in both cell lines. The bcl-2, p53 and cox-2 genes in both cell lines treated with ASA seem to exhibit different patterns of expression. However, normal lymphocytes treated with the same ASA concentrations were more resistant than tumoral cells. The results of this work show that both cell lines responded to treatment with ASA, demonstrating a possible antitumoral and anti-MDR role for this drug
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Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
(2010) Votto, Ana Paula de Souza; Domingues, Beatriz Spotorno; Souza, Michele Moraes de; Silva Júnior, Flávio Manoel Rodrigues da; Barbosa, Sergiane Caldas; Filgueira, Daza de Moraes Vaz Batista; Clementin, Rosilene Maria; Primel, Ednei Gilberto; Vallochi, Adriana Lima; Furlong, Eliana Badiale; Trindade, Gilma Santos
Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the Lucena cells experienced a significant increase of necrosis. An antioxidant capacity was verified for OE discarding oxidative damage. However, OE provoked similar significant DNA damage in both cell lines. Thus, the OE capacity to overcome the MDR phenotype suggests anti-MDR action of OE.