EQA - Escola de Química e Alimentos
URI permanente desta comunidadehttps://rihomolog.furg.br/handle/1/1610
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7 resultados
Resultados da Pesquisa
- ItemSpirulina as a protein source in the nutritional recovery of wistar rats(2013) Moreira, Lidiane Muniz; Behling, Bruna del Sacramento; Rodrigues, Rosane da Silva; Costa, Jorge Alberto Vieira; Soares, Leonor Almeida de SouzaThis study was aimed at evaluating the effects of Spirulina (Arthrospira) as a protein source in the nutritional recovery of rats subjected to protein malnutrition. In order to understand such effects, responses of the developing body, serum biochemical profile, and blood count of animals were analyzed. A total of, 23 Wistar Rattus norvegicus were given a proteic diet (AIN-93G adapted) in malnutrition phase (phase I) for 10 days. After this period, the rats were separated into four groups according to the protein source (recovery phase): Control (C), casein 12%; Spirulina level I (S1), Spirulina 8.8% + casein 5.0%; Spirulina level II (S2), Spirulina 17.6% + casein 0.15%, and Spirulina level III (S3), Spirulina 26.4%. Recovery phase (phase II) lasted 30 days. In both the phases, the consumption and body weight of the rats were monitored daily and weekly, respectively. The S1 treatment showed the highest values of Feed Efficiency Ratio (FER) and Quotient of Protein Efficiency Ratio (QPE) (p≤0.05). Significant differences among the treatments were found mainly in the levels of serum lipids, and S2 diet caused an increase in such responses (p≤0.05). These results demonstrated that the nutritional recovery of animals was enabled by different concentrations of Spirulina.
- ItemFatty acids profile of spirulina platensis grown under different temperatures and nitrogen concentrations(2004) Colla, Luciane Maria; Bertolin, Telma Elita; Costa, Jorge Alberto VieiraThe influence of culture temperature and the concentration of sodium nitrate (NaNO3) on the gas-chromatographic profile of the fatty acids of the filamentous cyanobacterium Spirulina platensis was evaluated. We found that temperature was the most important factor and that the greatest amount of gamma-linolenic acid (GLA) was obtained at 30 ∞C, the fatty acid profile of the Spirulina cultivated showing that (in order of abundance) palmitic, linolenic and linoleic acids were most prevalent.
- ItemPerfil de Ácidos Graxos de Microalgas Cultivadas Com Dióxido de Carbono(2008) Morais, Michele Greque de; Costa, Jorge Alberto VieiraAs microalgas são consideradas fontes potenciais de diversos compostos químicos. Os ácidos graxos obtidos da biomassa podem apresentar efeitos terapêuticos em humanos e podem ser usados para produção de biodiesel. Objetivou-se, neste trabalho verificar o conteúdo lipídico e o perfil dos ácidos graxos das microalgas Spirulina sp., Scenedesmus obliquus, Chlorella kessleri e Chlorella vulgaris cultivadas em diferentes concentrações de dióxido de carbono e bicarbonato de sódio. A microalga Chlorella kessleri cultivada com 12% de CO2 apresentou a maior concentração de lipídios na biomassa seca (9,7% p/p). A máxima concentração de ácidos graxos insaturados foi 72,0% (p/p) para C. vulgaris cultivada com 12% de CO2. Para os ácidos graxos saturados o maior valor encontrado foi 81,6% (p/p), quando a microalga Spirulina sp. foi cultivada com 18% de CO2 e 16,8 g.L-1 de bicarbonato de sódio.
- ItemEffect of Microalga Spirulina platensis (Arthrospira platensis) on Hippocampus Lipoperoxidation and Lipid Profile in Rats with Induced Hypercholesterolemia(2009) Bertolin, Telma Elita; Pilatti, Dayane; Giacomini, Ana Cristina Vendrametto Varrone; Bavaresco, Caren Serra; Colla, Luciane Maria; Costa, Jorge Alberto VieiraStudies have been conducted on microalga Spirulina platensis (Arthrospira platensis) due to its therapeutic potential in several areas, including the capacity for preventing and decreasing the damages caused by hyperlipidemia and the antioxidant activity. The aim of the study was to evaluate the effect of microalga Spirulina platensis on hippocampus lipoperoxidation and lipid profile in rats with induced hypercholesterolemia during 60 days. The measurement of hippocampus lipoperoxidation did not demonstrate significant difference (p>0.05) when Spirulina platensis was added to hypercholesterolemic diet. The evaluation of lipid profile showed that the administration of the microalga in therapeutic and preventive ways led to a significant protective effect (p<0.05) from hypercholesterolemia.
- ItemCultivation of microalgae Spirulina platensis (Arthrospira platensis) from biological treatment of swine wastewater(2010) Mezzomo, Natália; Saggiorato, Adriana Galon; Siebert, Rochele; Tatsch, Pihetra Oliveira; Lago, Maria Cristina; Hemkemeier, Marcelo; Costa, Jorge Alberto Vieira; Bertolin, Telma Elita; Colla, Luciane MariaThe microalgae biomass production from swine wastewater is a possible solution for the environmental impact generated by wastewater discharge into water sources. The biomass can be added to fish feed, which can be used in the formulation of meat products. This work addresses the adaptation of the microalgae Spirulina platensis (Arthrospira platensis) in swine wastewater and the study of the best dilution of the wastewater for maximum biomass production and for removal of Chemical Oxygen Demand (COD), ammonia and phosphorous to the microalgae. The cultivation of Spirulina platensis, strain Paracas presented maximum cellular concentrations and maximum specific growth rates in the wastewater concentration of 5.0 and 8.5%. The highest COD removals occurred with 26.5 and 30.0% of wastewater in the medium. The maximum removal of total phosphorous (41.6%), was with 8.5% of wastewater, which is related to the microalgae growth. The results of Spirulina culture in the swine wastewater demonstrated the possibility of using these microalgae for the COD and phosphorous removal and for biomass production.
- ItemCapacidade antioxidante da microalga Spirulina platensis em células da levedura Saccharomyces cerevisiae submetidas ao estressor paraquat(2010) Guarienti, Cíntia; Bertolin, Telma Elita; Costa, Jorge Alberto VieiraEm virtude de várias publicações terem mostrado a alta associação entre a geração de radicais livres e as doenças crônico-degenerativas, tem havido grande interesse por alimentos funcionais antioxidantes. O excesso de espécies reativas no organismo resulta em estresse oxidativo que provoca danos celulares e teciduais. A microalga Spirulina tem sido pesquisada em função de suas propriedades nutricionais e antioxidantes. O objetivo desse trabalho foi de avaliar a atividade antioxidante da microalga Spirulina, utilizando-se a levedura Saccharomyces cerevisiae como modelo biológico. A levedura foi submetida ao estressor 1,1’- dimetil - 4,4’- bipiridilio (paraquat), nas concentrações 0, 10 e 15 mM. O potencial antioxidante foi avaliado pela técnica de sobrevivência celular (plaqueamento) e pelo ensaio de lipoperoxidação (valores de TBA). Observou-se aumento significativo de sobrevivência celular (p ≤ 0,05), quando submetidos aos tratamentos com paraquat e Spirulina, em comparação com o experimento em que foi utilizado somente o paraquat. O agente estressor gerou aumento significativo (p ≤ 0,05) na lipoperoxidação (valores de TBA), o qual foi atenuado pelo tratamento com Spirulina, não diferindo do tratamento controle (p > 0,05). A microalga Spirulina apresentou capacidade antioxidante, que protege as células da levedura contra os danos oxidantes provocados pelo paraquat.
- ItemPilot scale semicontinuous production of spirulina biomass in southern Brazil(2009) Morais, Michele Greque de; Radmann, Elisangela Martha; Souza, Michele da Rosa Andrade Zimmermann de; Teixeira, Gilson Gonçalves; Brusch, Lucio Renato de Fraga; Costa, Jorge Alberto VieiraWe evaluated the feasibility of the pilot scale production of Spirulina strain LEB-18 in southern Brazil and assessed the quality of biomass produced in relation to its kinetics characteristic, nutritional value, heavy metal content and microbial content. The maximum mean biomass concentration was 1.24 g L− 1 and the maximum productivity was 69.16 g m− 2 d− 1. The biomass showed 84.0% digestibility, 86.0% (w/w) protein and 3.3% (w/w) lipid content. Analyses showed that the concentration (mg kg− 1) of heavy metals (As, 0.28 ± 0.01; Cd, < 0.05; Hg, < 0.01; and Pb, 0.17) and the microbial load (7.1 × 105 colony forming units per gram) were lower than the internationally accepted standards. These results show that pilot scale cultivation of Spirulina LEB-18 in southern Brazil is feasible and that the biomass produced is within the internationally recognized standards for use as a food additive for increasing the nutritional potential of conventional products.