EQA - Escola de Química e Alimentos
URI permanente desta comunidadehttps://rihomolog.furg.br/handle/1/1610
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2 resultados
Resultados da Pesquisa
- ItemIsolation and Characterization of a New Arthrospira Strain(2008) Morais, Michele Greque de; Reichert, Carolina da Cruz; Dalcanton, Francieli; Durante, Andrei José; Marins, Luis Fernando Fernandes; Costa, Jorge Alberto VieiraA filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol mÐ2 sÐ1 according to a complete 32 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μmax = 0.22 dÐ1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol mÐ2 sÐ1 and the highest protein content (86.0% w/w).
- ItemPilot scale semicontinuous production of spirulina biomass in southern Brazil(2009) Morais, Michele Greque de; Radmann, Elisangela Martha; Souza, Michele da Rosa Andrade Zimmermann de; Teixeira, Gilson Gonçalves; Brusch, Lucio Renato de Fraga; Costa, Jorge Alberto VieiraWe evaluated the feasibility of the pilot scale production of Spirulina strain LEB-18 in southern Brazil and assessed the quality of biomass produced in relation to its kinetics characteristic, nutritional value, heavy metal content and microbial content. The maximum mean biomass concentration was 1.24 g L− 1 and the maximum productivity was 69.16 g m− 2 d− 1. The biomass showed 84.0% digestibility, 86.0% (w/w) protein and 3.3% (w/w) lipid content. Analyses showed that the concentration (mg kg− 1) of heavy metals (As, 0.28 ± 0.01; Cd, < 0.05; Hg, < 0.01; and Pb, 0.17) and the microbial load (7.1 × 105 colony forming units per gram) were lower than the internationally accepted standards. These results show that pilot scale cultivation of Spirulina LEB-18 in southern Brazil is feasible and that the biomass produced is within the internationally recognized standards for use as a food additive for increasing the nutritional potential of conventional products.