FAMED – Faculdade de Medicina
URI permanente desta comunidadehttps://rihomolog.furg.br/handle/1/2422
Navegar
67 resultados
Resultados da Pesquisa
- ItemThe BACTEC MGIT™ 320 system as a laboratory tool to diagnose tuberculosis in a Brazilian hospital with a high prevalence of HIV infection(2016) Brum, Clarice Brinck; Soares, Daniela Fernandes Ramos; Abilleira, Fernanda de Souza; Silva, Ana Bárbara Scholante; Groll, Andrea Von; Silva, Pedro Eduardo Almeida daIntroduction: The World Health Organization endorses the BACTEC Mycobacterial Growth Indicator Tube (MGIT)™ system as a rapid, sensitive, and specifi c method to diagnostic of tuberculosis. Here, we compared the performance of this system against Ogawa-Kudoh cultures and microscopy. Methods: A total of 927 samples were obtained between December 2011 and December 2013 from 652 cases of suspected tuberculosis at the School Hospital of the Federal University of Rio Grande in Brazil. Results: The MGIT system confi rmed tuberculosis in more cases in less time. Conclusions: The MGIT system is an effective tool for early diagnosis of tuberculosis, especially in patients with HIV/AIDS.
- ItemConvenient One-Pot Synthesis of N-Substituted 3-Trifluoroacetyl Pyrroles(2009) Zanatta, Nilo; Wouters, Ana Dionéia; Fantinel, Leonardo; Silva, Fábio de Moraes Vaz da; Barichello, Rosemário; Silva, Pedro Eduardo Almeida da; Soares, Daniela Fernandes Ramos; Bonacorso, Helio Gauze; Martins, Marcos Antonio PintoA new one-pot strategy for the synthesis of a series of new N-substituted 3-trifluoroacetyl pyrroles is presented. These compounds were obtained by the reaction of 3-trifluoroacetyl-4,5- dihydrofuran with primary amines, which generated 1,1,1-trifluoro- 3-(2-hydroxyethyl)-4-alkylaminobut-3-en-2-one intermediates. In most cases these intermediates were not stable enough to be isolated. Thus, in the same reaction vessel they were directly submitted to oxidation with PCC (Corey’s reagent) to furnish 1,1,1-trifluoro- 3-(2-ethanal)-4-alkylaminobut-3-en-2-ones, which under reflux underwent intramolecular cyclization to give the desired N-substituted 3-trifluoroacetyl pyrroles, in moderate yields. All of these pyrroles were tested against pan-susceptible Mycobacterium tuberculosis H37Rv and clinical isolates INH- and RMP-resistant strain and some of these compounds showed significant in vitro antimicrobial activity.
- ItemTriterpenes and the Antimycobacterial Activity of Duroia macrophylla Huber (Rubiaceae)(2013) Martins, Daiane; Carrion, Lillian Lucas; Soares, Daniela Fernandes Ramos; Salomé, Kahlil Schwanka; Silva, Pedro Eduardo Almeida da; Barison, Andersson; Nunez, Cecilia VeronicaDuroia macrophylla popularly known as “cabec¸a-de-urubu,” “apuru ´ ´ı,” or “puru´ı-grande-da-mata” occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in all dichloromethane and methanol extracts, respectively. Methanol extracts from both branches (1st collection) and leaves (2nd collection) presented hydrolyzed tannins, yet alkaloids were only detected in the dichloromethane and methanol extracts from branches at the 2nd collection. Phenol compounds were found in both dichloromethane extracts’ collections. The action of every extract was assayed against Mycobacterium tuberculosis (RMPr, H37Rv, and INHr strains), showing that the dichloromethane extract from leaves (1st collection) has the major biological activity, with a MIC of 6.25 𝜇��g/mL for the INHr strain, 25.0 𝜇��g/mL for the RMPr strain, and ≤6.25 𝜇��g/mL for the H37Rv strain. The chromatographic fractioning of the dichloromethane extract from leaves (1st collection) yielded the isolation of two triterpenes: oleanolic and ursolic acids, which were identified by NMR analysis and reported for the first time in the Duroia genus.
- ItemDiagnosis of bovine tuberculosis: review of main techniques(2015) Fernandes, Ramos, Daniela; Silva, Pedro Eduardo Almeida da; Dellagostin, Odir AntonioBovine tuberculosis (BTB) remains an important economic and zoonotic problem in Latin America. Traditionally, the fight against BTB is initiated by the implementation of routine diagnostic tests for certification of free properties. The diagnosis of BTB can be made by direct and indirect methods, in which we can mention clinical, post mortem, histopathological, immunological, bacteriological and molecular methods. The renewal of scientific interest in tuberculosis in recent year has led to develop and improve methods of diagnosis, prevention, control and eradication of BTB. The aim of this review is to present and discuss different diagnosis methods of BTB.
- ItemMolecular typing of Mycobacterium bovis isolates: A review(2014) Soares, Daniela Fernandes Ramos; Tavares, Lucas; Silva, Pedro Eduardo Almeida da; Dellagostin, Odir AntonioMycobacterium bovis is the main causative agent of animal tuberculosis (TB) and it may cause TB in humans. Molecular typing of M. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. Techniques used for typing M. bovis have evolved over the last 2 decades, and PCR-based methods such as spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) have been extensively used. These techniques can provide epidemiological information about isolates of M. Bovis that may help control bovine TB by indicating possible links between diseased animals, detecting and sampling outbreaks, and even demonstrating cases of laboratory cross-contamination between samples. This review will focus on techniques used for the molecular typing of M. bovis and discuss their general aspects and applications.
- ItemPreparative Isolation of Antimycobacterial Shoreic Acid from Cabralea canjerana by High Speed Countercurrent Chromatography(2008) Leitão, Gilda Guimarães; Abreu, Lisandra Ferreira de; Costa, Fernanda das Neves; Brum, Thiago Braga; Soares, Daniela Fernandes Ramos; Silva, Pedro Eduardo Almeida da; Lourenço, Maria Cristina da Silva; Leitão, Suzana GuimarãesHigh speed countercurrent chromatography (HSCCC) was used to isolate the dammarane type triterpene shoreic acid from the dichloromethane extract of leaves of Cabralea canjerana, which showed activity against Mycobacterium tuberculosis. A preparative scale-up of the process was also developed.
- ItemAnthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae(2015) Santos, Taís Mata dos; Pinto, Nitza França; Santos, Hílton Antônio Mata dos; Moura, Kelly Cristina Gallan de; Carneiro, Paula Fernandes; Carvalho, Tatiane dos Santos; Del Rio, Karina Pena; Pinto, Maria do Carmo Freire Ribeiro; Martins, Lourdes Helena Rodrigues; Fenalti, Juliana Montelli; Silva, Pedro Eduardo Almeida da; Scaini, Carlos JamesAnthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitroactivity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds.
- ItemOn the search for potential antimycobacterial drugs: synthesis of naphthoquinoidal, phenazinic and 1,2,3-triazolic compounds and evaluation against Mycobacterium tuberculosis(2015) Jardim, Guilherme Augusto de Melo; Cruz, Eduardo Henrique Guimarães da; Valença, Wagner; Resende, Jarbas Magalhães; Rodrigues, Bernardo Lages; Soares, Daniela Fernandes Ramos; Oliveira, Ronaldo; Silva, Pedro Eduardo Almeida da; Silva Júnior, Eufrânio Nunes daFifteen naphthoquinones, sixteen phenazines and fifteen aryl triazoles were synthesized and evaluated against Mycobacterium tuberculosis. Twenty five substances are reported here for the first time and, among all of the compounds evaluated, six presented MIC (minimal inhibitory concentration) values ≤ 6.25 μg mL-1. These substances are promising antimycobacterial prototypes.
- ItemAntimycobacterial activity of usnic acid against resistant and susceptible strains of Mycobacterium tuberculosis and non-tuberculous mycobacteria(2010) Soares, Daniela Fernandes Ramos; Silva, Pedro Eduardo Almeida daTuberculosis remains a serious public health problem, with nine million cases being reported annually. Treatment with antibiotics is the most effective mechanism to control this disease, although the increase in cases with resistant strains, co-infection with HIV, and the long duration of treatment has established the need to develop new drugs. Here we show the activity of usnic acid against susceptible and resistant Mycobacterium tuberculosis strains and against nontuberculous mycobacteria. Further, we did not identify any contribution of efflux in innate resistance to usnic acid.
- ItemAvaliação da atividade antimicobacteriana de extratos vegetais e moléculas de síntese(2008) Soares, Daniela Fernandes Ramos; Groll, Andrea Von; Ramis, Ivy Bastos; Coelho, Tatiane Silveira; Flach, Juliana; Silva, Pedro Eduardo Almeida daA tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de casos. Considerando o aumento no número de casos por cepas multidrogarresistente (MDR-TB) e o reduzido arsenal terapêutico, faz-se necessária à busca de novas alternativas farmacológicas para o tratamento de TB. Os extratos vegetais tem sido uma fonte importante para o desenvolvimento de substâncias com atividade antimicrobiana, principalmente, em países ricos em biodiversidade, como é o caso do Brasil. Outra estrategia é a síntese de novas moléculas que podem ser desenhadas a partir de estruturas já conhecidas ou buscando atingir novos alvos. O grupo de pesquisa do Laboratório de Micobactérias/Biologia Molecular da FURG tem avaliado a atividade antibacteriana de extratos vegetais e moléculas sintéticas buscando novas alternativas terapêuticas para TB.