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dc.contributor.author Gouveia, Glauce Ribeiro
dc.contributor.author Marques, Daiane
dc.contributor.author Cruz, Bruno Pinto
dc.contributor.author Monserrat, Laura Alicia Geracitano
dc.contributor.author Nery, Luiz Eduardo Maia
dc.contributor.author Trindade, Gilma Santos
dc.date.accessioned 2011-11-11T23:11:29Z
dc.date.available 2011-11-11T23:11:29Z
dc.date.issued 2005
dc.identifier.citation GOUVEIA, G.. R. et al. Antioxidant defenses and DNA damage induced by UVA and UVB radiation in the crab Chasmagnathus granulata (Decapoda, Brachyura). Photochemistry and Photobiology, v. 81, p. 398-403, 2005. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2005.tb00200.x/abstract>. Acesso em: 10 abr. 2011. pt_BR
dc.identifier.issn 1011-1344
dc.identifier.uri http://repositorio.furg.br/handle/1/1346
dc.description.abstract The photoprotector role of pigment dispersion in the melanophores of the crab, Chasmagnathus granulata, against DNA and oxidative damages caused by UV-A and UV-B was investigated.Intact and eyestalkless crabs were used. In eyestalkless crabs,the dorsal epidermis of the cephalothorax (dispersed melanophores)and the epidermis of pereiopods aggregated melanophores)were analyzed. Intact crabs showed only dispersed melanophores in the two epidermis. Antioxidant enzymes activity and lipoperoxidation content were analyzed after UV-A (2.5 J/cm2) or UV-B (8.6 J/cm2) irradiation. DNA damage was analyzed by single cell electrophoresis (comet) assay, after exposure to UV-B (8.6 J/cm2). UV-A radiation increased the glutatione-S-transferase activity in the pereiopods epidermis of eyestalkless crabs (P < 0.05). UV-B radiation induced DNA damage in the dorsal epidermis of eyestalkless crabs (P < 0.05). In pereiopod epidermis of eyestalkless crabs, there was no significant difference between control and UV-B–exposed crabs. In the pereiopods epidermis of eyestalkless, the control group showed higher scores of DNA damage and ~50% of cellular viability. Because in eyestalkless and irradiated crabs the cellular viability was ~5%, it was not possible to observe nuclei for determination of DNA damage. The findings show that melanophores can play a role in the defense against harmful effects of a momentary exposure to UV radiation. pt_BR
dc.language.iso eng pt_BR
dc.rights restrict access pt_BR
dc.subject Antioxidant defenses pt_BR
dc.subject UV-A and UV-B pt_BR
dc.subject Chasmagnathus granulata pt_BR
dc.subject DNA pt_BR
dc.title Antioxidant Defenses and DNA Damage Induced by UV-A and UV-B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura) pt_BR
dc.type article pt_BR
dc.identifier.doi 10.1562/2004-05-26-RA-179.1 pt_BR


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