Relationship between multidrug resistance (MDR) and stem cell markers in human chronic myeloid leukemia cell lines
Marques, Daiane; Sandrini, Juliana Zomer; Boyle, Robert Tew; Marins, Luis Fernando Fernandes; Trindade, Gilma Santos
Abstract:
The K562 cell line (chronic myeloid leukemia), sensitive to chemotherapy (non-MDR), and the Lucena cell line, resistant to chemotherapy (MDR) were investigated. The results suggest that both cell lines possess CD34 + CD38 − profiles of hematopoietic stem cell markers. The promoter regions of ABCB1, ABCG2 and ABCC1 genes contain binding sites for the Oct-4 transcripton factor,which is also considered a marker of tumor stem cells. Lucena cells showed an over-expression of the ABCB1 gene and a high expression of the Oct-4, ABCG2 and ABCC1 genes as compared to K562 cells.