Validação de método para determinação de ácidos orgânicos voláteis em efluentes de reatores anaeróbios empregando cromatografia líquida

Cerqueira, Maristela Barnes Rodrigues; Dias, Adriana Neves; Barbosa, Sergiane Caldas; Santana, Fabricio Butierres; D'Oca, Marcelo Gonçalves Montes; Primel, Ednei Gilberto


This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r2>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor.

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  • EQA - Artigos Publicados em Periódicos