Show simple item record Fonseca, Renata Aline dos Santos da Rafael, Ruan da Silva Kalil, Susana Juliano Burkert, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros 2012-03-24T00:03:46Z 2012-03-24T00:03:46Z 2010
dc.identifier.citation FONSECA, Renata Aline dos Santos da et al. Different cell disruption methods for astaxanthin recovery by Phaffia rhodozyma.African Journal of Biotechnology, v. 10, n. 7, p.1165-1171, 2010. Disponível em: <>. Acesso em: 19 dez. 2011. pt_BR
dc.identifier.issn 1684–5315
dc.description.abstract Astaxanthin (3,3'-dihydroxy-b,b'-carotene-4,4'-dione) is carotenoid of high market value whose demand has increased in such fields as aquaculture, pharmaceutical supplements and natural coloring. Cell disruption is the first step for isolating intracellular materials and it depends on the cell wall permeability. In order to maximize the recovery of astaxanthin from Phaffia rhodozyma NRRL-Y17268, drying and freeze pretreatments were tested by different cell disruption methods: abrasion with celite, glass pearls in vortex agitator, ultrasonic waves, sodium carbonate (Na2CO3) and dimethyl sulfoxide (DMSO). The method with Na2CO3 was not effective; meanwhile, the agitator with glass pearls, the abrasion with celite and the ultrasonic waves were found as promising for future studies. As a result, the DMSO in freeze-dried biomass with 4 process cycles and biomass/DMSO relation of 0.025 g/ml was found to be the most efficient for analytical determination, increasing about up to 25 times the astaxanthin recovery. pt_BR
dc.language.iso por pt_BR
dc.rights open access pt_BR
dc.subject Carotenoids pt_BR
dc.subject Yeast pt_BR
dc.subject Chemical disruption pt_BR
dc.subject Dimethyl sulfoxide pt_BR
dc.title Different cell disruption methods for astaxanthin recovery by Phaffia rhodozyma pt_BR
dc.type article pt_BR

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