dc.contributor.author |
Marins, Luis Fernando Fernandes |
|
dc.contributor.author |
Iyengar, Arati |
|
dc.contributor.author |
Maclean, Norman |
|
dc.contributor.author |
Sabaj, José Alberto Levy |
|
dc.contributor.author |
Sohm, Frédéric |
|
dc.date.accessioned |
2012-11-22T00:41:18Z |
|
dc.date.available |
2012-11-22T00:41:18Z |
|
dc.date.issued |
2002 |
|
dc.identifier.citation |
MARINS, Luis Fernando Fernandes et al. Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos. Genetics and Molecular Biology, v. 25, n.3, p. 293-298, 2002. Disponível em: <http://www.scielo.br/pdf/gmb/v25n3/a07v25n3.pdf>. Acesso em: 10 nov. 2012. |
pt_BR |
dc.identifier.issn |
1415-4757 |
|
dc.identifier.uri |
http://repositorio.furg.br/handle/1/2807 |
|
dc.description.abstract |
In this study, we describe the use of a STAT5 responsive element (LHRE) reporter gene to monitor the activity of the growth hormone (GH) transduction pathway following expression of heterologous fish GH and rat STAT5b in tilapiaembryos and fish fibroblast cells. Our results indicate that both GH and STAT5b are able to activate the LHRE at high
levels when transferred separately, demonstrating the substantial level of conservation of the GH signal transduction
pathways between fish and mammals. Unexpectedly, co-expression experiments show a strong inhibition of the GH-dependent activation, suggesting that simultaneous GH and STAT5b overexpression can counteract effects of GH expression in tilapia embryos. |
pt_BR |
dc.language.iso |
eng |
pt_BR |
dc.rights |
open access |
pt_BR |
dc.subject |
Teleost |
pt_BR |
dc.subject |
Growth hormone |
pt_BR |
dc.subject |
Signal transduction |
pt_BR |
dc.subject |
Cytokine |
pt_BR |
dc.subject |
Trangenesis signal transducers and activators of transcription |
pt_BR |
dc.title |
Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos |
pt_BR |
dc.type |
article |
pt_BR |
dc.identifier.doi |
http://dx.doi.org/10.1590/S1415-47572002000300007 |
pt_BR |