Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

Santos, Elisane Odriosolla dos; Michelon, Mariano; Furlong, Eliana Badiale; Burkert, Janaina Fernandes de Medeiros; Kalil, Susana Juliano; Burkert, Carlos André Veiga

Abstract:

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

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