Show simple item record Burkert, Janaina Fernandes de Medeiros Kalil, Susana Juliano Maugeri Filho, Francisco Rodrigues, Maria Isabel 2014-09-18T11:47:10Z 2014-09-18T11:47:10Z 2006
dc.identifier.citation BURKERT, Janaína Fernandes de Medeiros et al. Parameters optimization for enzymatic assays using experimental design. Brazilian Journal of Chemical Engineering, v. 23, n. 2, p. 163-170, 2006. Disponível em: <>. Acesso em: 16 set. 2014. pt_BR
dc.identifier.issn 0104-6632
dc.description.abstract The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C. pt_BR
dc.language.iso eng pt_BR
dc.rights open access pt_BR
dc.subject Inulinase pt_BR
dc.subject Kluyveromyces marxianus pt_BR
dc.subject Activity pt_BR
dc.subject Factorial design pt_BR
dc.subject Response surface analysis pt_BR
dc.title Parameters optimization for enzymatic assays using experimental design pt_BR
dc.type article pt_BR

Files in this item

This item appears in the following Collection(s)


  • EQA - Artigos Publicados em Periódicos
  • Show simple item record