Solid State Biosurfactant Production in a Fixed-Bed Column Bioreactor

Abstract

Biosurfactants are surface active substances which reduce interfacial tension and are produced or excreted at the microbial cell surface. We evaluated the biosurfactant production by Aspergillus fumigatus and Phialemonium sp. in solid state processes using fixed-bed column reactors. We evaluated two media, rice husks alone (simple support) and rice husks plus defatted rice bran (complex support), both enriched with either soy oil or diesel oil. The highest water-in-oil emulsifying activity (EAw/o) obtained was 7.36 EU gÐ1 produced by A. fumigatus growing on complex support enriched with soy oil and supplied with air at a rate of 60 mL gÐ1 hÐ1, while Phialemonium sp. had a maximum production of 6.11 EU gÐ1 using the simple support with diesel oil and an aeration rate of 120 mL gÐ1 hÐ1. The highest oilin-water emulsifying activity (EAo/w) was 12.21 EU gÐ1 produced by Phialemonium sp. on the complex support enriched with diesel oil and at an aeration rate of 60 mL gÐ1 hÐ1, while A. fumigatus produced a maximum EAo/w of 10.98 EU gÐ1 when growing on the complex support with no additional carbon source and an aeration rate of 60 mL gÐ1 hÐ1.