Antioxidant properties of the mucus secreted by Laeonereis acuta (Polychaeta, Nereididae): a defense against environmental pro-oxidants?

Abstract

Polychaeta species like Laeonereis acuta (Nereididae) usually secrete great amounts of mucus that wrap the animal inside. Taking into account that fungi action in the sediment and UV radiation acting on dissolved organic matter in the water produces reactive oxygen species (ROS) like hydrogen peroxide (H2O2), it was considered that the mucus secretion could represent an antioxidant defense against environmental ROS. Antioxidant enzymes (catalase—CAT; superoxide dismutase—SOD; glutathione peroxidase—GPx and glutathione-S-transferase—GST) and total antioxidant capacity (TOSC) were determined in worms and mucus secretion. Higher (pb0.05) CAT, GPx and TOSC values were registered in mucus samples respect worms, SOD activity was similar (pN0.05) in both kind of samples, and absence of GST activity was observed in mucus samples, suggesting absence of catalyzed phase II reactions. In assays conducted with hepatoma cell lines exposed to H2O2, it was verified that:(1) mucus co-exposure significantly (pb0.05) lowered DNA damage induced by H2O2; (2) ROS production was significantly (pb0.05) reduced when cells were exposed simultaneously with mucus samples and H2O2 respect H2O2 alone. It can be concluded that the mucus production contributes substantially to the antioxidant defense system of the worm against environmental ROS through the interception or degradation of H2O2, peroxyl and hydroxyl radicals.